摘要
目的：为适应乳腺癌诊断与治疗的进展，通过提供客观的形态学依据，以达到最佳治疗的目的。方法：对１４０例妇女乳腺癌根治术标本根据肿瘤始发组织学部位、形态特点、分化程度、组织结构、肿瘤进展的动态过程、形态与功能变异、瘤细胞核ＤＮＡ含量及生物学行为进行了组织学分类。部分切片采用Ｓ－Ｐ法免疫组化染α－ｓｍｏｏｔｈｍｕｓｃｌｅａｃｔｉｎ（α－ＳＭＡ），观察导管肌上皮、基底膜破坏和癌巢周围肌纤维母细胞反应性增生用以辅助分类，９０例不同组织类型的癌切片做Ｆｅｕｌｇｅｎ染色，用ＣＭＩＡＳⅡ图像分析观察瘤细胞核ＤＮＡ含量。结果：把乳腺癌分成导管癌、小叶癌、化生性癌和特殊类型的癌４大类，根据肿瘤连续进展的动态过程和组织结构每类又分成若干小类。不同组织类型瘤细胞ＤＮＡ含量统计学处理差异显著，提示每组类型有各自生物学的独特性，分类合理。
Purpose In order to adapt progress of diagnosis and treatment for breast carcinoma and provide morphological basis and attain best treatments goal on treatment of breast carcinoma. Methods The histological classification was performed with 140 radical mastectomy specimens in female. The classification was according to the histological origination of tumor cells, morphological characteristics, degree of carcinoma progress, paramorphia and dysfunction, DNA content of tumor cells and biobehaviour. A part of slices were stained by αsmooth muscle actin with SP method of immunohistochemistry so as to observe destruction of duct base membrane and myoepithelium, and proliferative reaction of myofibroblasts around carcinoma nests. In order to analyze DNA content of tumor cells, the tissue slices stained Feulgen were studied using CMIAS Ⅱ in 90 cases of a great variety types of breast carcinoma. Results The breast carcinoma was divided into duct carcinoma, lobular carcinoma, netaplastic carcinoma, and particular type carcinoma, which was subdivided into some subtypical carcinoma. There were statistical significant difference in DNA content in the tumor cells of various histological type. Conclusions Its suggested that each type has oneself biological independent character and that the classification is rational. The morphological classification of breast carcinoma lay a morphological foundation for choosing of clinical treatment.
出处
《临床与实验病理学杂志》
CAS
CSCD
1998年第1期5-8,I002,共5页
Chinese Journal of Clinical and Experimental Pathology
关键词
病理学
分类
DNA
乳腺癌
breast neoplasms
pathologoical classification
DNA, neoplasms
image interpretation, computerassisted
