摘要
123例乳腺疾病(良性42例、恶性81例)经免疫组化ABC法应用抗肌动蛋白HHF_(35)、抗细胞角蛋白MAK—6和AE_1—AE_3、抗Ⅳ型胶原及抗层粘连蛋白等5种抗体对肌上皮细胞(MC)和基底膜(BM)进行标记,并对其中15例作透射电镜观察。结果发现乳腺各种良性疾病的导管及乳头状结构多有连续的MC和BM,而相应的各种乳腺癌中MC和BM多消失,MC和BM两者呈正相关。因此,MC和BM对良、恶性疾病的鉴别诊断以及原位癌早期浸润的识别有一定的意义。
Using an immunohistochemical ABC method, authors studied 123 human breast diseases (benign 42 cases, malignant 81 eases) with MAK—6, AE_1—AE_3(monoclonal antibodies against cytokeratin),HHF—35 (monoclonal antibody against muscle actins) and monoclonal antibody against type Ⅳ collagen as well as polyclonal antibody against laminin to mark myoepithelial cells(MC) and basement membrane (BM). Of 15 cases observed with transmission electron microscopy,the results showed that MC and BM usually were continuous in the ducts, acini and papillary archiecture in bengin lesions, while discontinuous and even disappearing in malignant tumors. In situ carcinomas, BM disruption and MC disappearence often presuppose invasion occurance. Therefore, MC and BM give valuable information in differential diagnosis in breast pathology.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
1992年第2期85-89,共5页
Chinese Journal of Clinical and Experimental Pathology
关键词
肌上皮细胞
基底膜
乳腺肿瘤癌
myoepithelial cell
basement membrane
immunohistochemistry breast lesion
