摘要
以海雀稗品种Adalay(Paspalum vaginatum Sw.cv.Adalay)的颗粒状胚性愈伤组织为材料,在离体培养条件下研究不同剂量的60Coγ射线辐照对愈伤组织绿苗分化和植株再生的影响。结果显示,辐照剂量在20～50Gy时,辐照处理的愈伤组织分化绿苗的频率从94%左右降至85%;剂量在60～80Gy时,愈伤组织的绿苗分化率从76%左右降至30%左右,相对植株再生率从44.5%降至8.7%。60Gy为辐照处理海雀稗颗粒状愈伤组织的适宜剂量,辐照处理的愈伤组织在附加BAP2.0mg/L的愈伤组织分化培养基上,绿苗分化率在76%左右,分化的绿苗转到附加NAA0.5mg/L的生根培养基上,相对植株再生率为44.5%。SRAP分子标记测定结果显示,特异性SRAP标记与体细胞辐照诱变突变体相关。本研究建立起海雀稗体细胞60Coγ射线辐照诱变技术,为在细胞水平上开展海雀稗辐照诱变育种提供条件。
The pellet embryonic calli of Paspalum vagiantum Sw.cv.Adalay were used for 60 Co γ-rays irradiation.In the callus subculture medium with 2,4-D 2.0mg /L and BAP 0.05mg /L,calli were irradiated at the dose rate 1 Gy /min of 60Co γ-rays.Results showed that the rate of shoot regeneration was from 94% to 85% when calli irradiated with 20 ～ 50 Gy;the rate of shoot regeneration was from 76% to 30% ,and the rate of plant regeneration was from 44.5% to 8.7% between 60 ～ 80 Gy.By 60 Gy of 60 Co γ-rays irradiation,the rate of shoot regeneration was about 76% in the differentiation medium with BAP 2.0mg /L,and the relative rate of plant regeneration was 44.5% in the rooting medium with NAA 0.5mg /L.The result of sequence-related amplified polymorphism（SRAP） markers analysis showed that the specific SRAP markers were associated with the somatic mutants.The mutation technique of 60 Co γ-rays has been established in the somatic cell of Paspalum vaginatum Sw.
出处
《核农学报》
CAS
CSCD
北大核心
2010年第2期259-263,共5页
Journal of Nuclear Agricultural Sciences
基金
江苏省科技厅科技攻关项目(BE2003369)
