摘要
为筛选出具有潜在研究价值的优良性状葡萄植株,以鄞红葡萄及其8个优良单株5-1、5-2、10-1、10-2、20-1、20-2、40-1和40-2为研究材料,观测实生后代叶片、枝条和果实等主要形态特征和性状差异,并以其幼叶为材料,采用SSR分子标记技术分析8个优良单株与亲本鄞红之间的遗传差异。形态观测结果表明,5-2、10-2、20-1和20-2与亲本在果实成熟期、叶片形态和果皮色泽方面皆存在较大差异。分子标记结果表明,使用筛选出的24条带型清晰、多态性较高的引物,共扩增出237条带,多态性条带219条,平均多态性位点百分率为92.5%。聚类结果显示,实生后代和亲本遗传相似系数变化范围为0.60-0.79,在遗传相似系数0.65处,9份葡萄材料分为两大类群,第1类包括10-2和20-1,第2类包括5-2、20-2、5-1、10-1、40-1、40-2和鄞红;其中,5-2、10-2、20-1和20-2与亲本遗传相似系数较低,遗传差异较大,是早熟、外观品质优良的株系。本研究结果对丰富我国南方葡萄品种多样性具有重要意义。
To screen plants with excellent characteristics of potential applied value, Yinhong grape and its eight elite individuals i. e. 5 - 1, 5 -2, 10 - 1, 10 -2, 20 - 1, 20 -2, 40 - 1, 40 -2 were used as material to investigate major morphological characteristics and trait differences including leaves, branches and fruits. Genetic differences of the eight elite individuals and their parent Yinhong were analyzed by simple sequence repeat （SSR） markers. Morphological observations showed that with there were larger differences in maturity, leaf morphology and skin color between 5 - 2, 10 -2, 20 - 1, 20 - 2 and parent Yinhong. The results of molecular markers showed that twenty-four highly efficient amplification primers obtained from 122 arbitrary primers, total 219 out of 237 bands amplified showed polymorphisms, and the mean ratio of polymorphism was 93%. The clustering analysis showed that the genetic similarity coefficient among the seedlings and their parent ranged from 0.60 to 0.79. At the point of 0.65, 9 samples were classified into two groups. The first group included 10-2 and 20-1, the second group included5-2, 20-2, 5-1, 10-1,40-1,40 -2 and parent Yinhong. It was found that in genetic similarity coeffecient between individuals 5 -2, 10 -2, 20 - 1 and 20 -2 were lower and the degree of variation was larger. Individuals 5 -2, 10 -2, 20 - 1 and 20 -2 had excellent strains with premature, excellent appearance quality.
出处
《核农学报》
CAS
CSCD
北大核心
2016年第9期1684-1692,共9页
Journal of Nuclear Agricultural Sciences
基金
宁波市科技创新团队项目(2011B82019)
浙江省科技厅项目(2013C24002)
浙江省自然基金项目(Y13C150014)
关键词
葡萄
优良单株
遗传差异
SSR
Vitis vinifera L. , elite individuals, genetic differences, SSR markers
