摘要
探讨辐射损伤对粘膜免疫的影响。方法小鼠全身照射８Ｇｙ后，分离培养小肠上皮内淋巴细胞，并对其数量、细胞增殖活力、细胞毒力、ＴＮＦ－α和ＴＧＦ－β分泌水平进行检测。结果小肠上皮内淋巴细胞数量明显下降，增殖活力和细胞毒力明显受抑，都表现为伤后８小时下降，７２小时达最低值。上皮内淋巴细胞分泌ＴＮＦ－α和ＴＧＦ－β显著增多，表现为伤后８小时增高，７２小时达峰值。结论小肠上皮内淋巴细胞数量减少和功能降低成为全身放射损伤时造成肠粘膜免疫屏障功能受损的一个重要原因。
Objective\ To explore the characteristics of intestinal mucosal immunity after radiation injury. Methods\ Number, proliferation activity, cytotoxicity of IEL as well as the TNF α and TGF β concentrations in supernatant of cultured IELs were studied using freshly isolated IELs from whole small intestine of Kunming strain mice whole body irradiated with 8Gy 60 Co rays.Results\ The proliferation activity, cytotoxicity as well as the number of IELs in small intestinal mucosa were significantly decreased from 8h and reached the lowest level at 72h post irradiation. The TNF α and TGF β concentrations in supernatant of cultured IELs isolated from irradiated mice elevated at 8h and reached the peak values at 72h.Conclusion\ The decrease in number and important functions of IELs might be one of the reasons which damage the intestinal mucosal immunity barrier after whole body irradiation.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1998年第6期373-376,共4页
Chinese Journal of Radiological Medicine and Protection
基金
全军"九五"指令性攻关课题资助
关键词
辐射
肠上皮内
淋巴细胞增殖
细胞毒
Radiation\ \ Intraepithelial lymphocytes\ \ Cell proliferation \ \ Cytotoxine
