摘要
【目的】观察钩吻素子(Kou)对小鼠的抗应激作用。【方法】昆明种小鼠随机分为模型对照组,Kou低、中、高剂量组(剂量分别为1.2、2.4、4.8 mg.kg-1.d-1),各组按设计剂量灌胃给药,连续7 d;末次给药1 h后进行负重游泳、耐缺氧、耐高温及耐低温的应激实验,并检测小鼠血清超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量的变化。【结果】给予Kou处理后,在不同的抗应激实验中用药小鼠的生存时间均比模型对照组延长(P<0.05或P<0.01),在负重游泳实验中用药小鼠血清SOD活力上升、MDA含量下降(均P<0.01);但在小鼠耐高温实验中,Kou给药组和模型对照组的生存时间差异无统计学意义(P>0.05)。【结论】Kou能提高小鼠负重游泳、耐寒及耐缺氧能力,其抗应激作用可能与抗脂质过氧化有关。
[Objective] To observe the anti-stress effect of kotanine （Kou） on mice. [Methods] Kunming mice were randomized into model group, low-, moderate- and high-dose koumine （ 1.2, 2.4, and 4.8mg·kg^-1·d^-1） groups. The treatment lasted 7 days. One hour after last administration, stress tests such as weight-bearing swimming, antihypoxia, high-temperature resistance and low-temperature resistance were carried out. The changes of serum superoxide dismutase （SOD） activity and malondialdehyde （MDA） content were also observed. [ Results] During the anti-stress tests, the survival time was prolonged in koumine groups as compared with the model group （ P 〈 0.05 or P 〈 0.01）. In the weight-bearing swimming test, serum SOD activity was increased and MDA conent was decreased in koumine groups as compared with the model group （ P 〈 0.01 ）. However, in mice high-temperature resistance test, the difference of survival time was insignificant between koumine groups and model group （ P 〉 0.05）. [ Conclusion ] Koumine can increase the mice tolerance of weight-bearing swimming, cold and hypoxia, and its anti-stress mechanism may be related to the antilipid peroxidation.
出处
《广州中医药大学学报》
CAS
2007年第4期317-319,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省重点攻关项目(编号:2002A301030108
2003C20515)
