摘要
目的探索肠上皮内淋巴细胞（IEL）受照射后IFN-γ分泌的变化及其对肠上皮细胞增殖的影响。方法采用ELISPOT法观察IEL受^60Coγ射线8Gy照射前后IFN-γ的分泌变化；利用新分离的IEL及大鼠隐窝空肠上皮细胞株（IEC-6）为离体细胞模型，通过混合、双室（隔离）培养系统，用MTF法观察IEL对IEC-6增殖活性的影响。结果照射后IEL分泌IFN-γ增加，受照射IEL培养上清对IEC-6细胞增殖具有抑制作用，而双室培养可减轻抑制作用，加入抗IFN-γ抗体亦可减轻对IEC-6的抑制作用。结论IEL受照后IFN-γ的分泌增加，对IEC-6的增殖具有抑制作用，IFN-γ是一种重要的介质因子，细胞与细胞紧密接触是IEL影响IEC-6增殖分化的一种重要方式。
Objective To explore the changes in IFN-γ secretion in intestinal intraepithelial lymphocytes （IEL） after radiation with 6o Co γ-ray of 8 Gy and its influence on the proliferation of IEC-6. Methods ELISPOT was used to detect the changes of IFN-γ secretion in IEL after radiation. The influence of IEL on the proliferation of crypt cell line from rat jejunal epithelium （IEC-6） was analyzed by MTT assay, using the cell models of IEC-6 and incorporate- and double-cell culture system. Results IFN-γ secretion in IEL after radiation was increased. And the culture supernatant of IEL after radiation had inhibitory action on the proliferation of IEC-6. The double-cell culture system could decrease this inhibitory action, and adding anti- IFN- γ have the same effect. Conclusions IFN-γ secretion in intestinal intraepithelial lymphocytes is enhanced after radiation and it has the inhibitory action on the the proliferation of IEC-6. Cell to cell contact may be an important way for IEL to inhibit the proliferation of IEC-6.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2007年第6期537-540,共4页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金资助项目（30270422）,国家自然科学基金重点资助项目（30230360）
关键词
辐射
肠上皮内淋巴细胞
肠上皮细胞
IFN-Γ
Irradiation
Intestinal intraepithelial lymphocytes （IEL）
Rat jejunal epithelium （IEC- 6）
IFN-γ
