摘要
背景:冻存脐带间充质干细胞,并有效地保持其生物学特性,是储存脐带间充质干细胞以供临床使用的重要工艺之一。目的:观察冻存后脐带间充质干细胞的生物学特性,验证其是否仍具有间充质干细胞的基本特征。方法:从脐带分离得到间充质干细胞后,将其冻存于液氮之中。比较经冻存复苏后的脐带间充质干细胞和新鲜制备的脐带间充质干细胞活率、抑制人外周血单个核细胞分泌γ-干扰素等方面的异同。检验经冻存复苏后的脐带间充质干细胞是否具有多向分化潜能,其表型是否满足间充质干细胞的基本特征。结果与结论:在细胞活率和抑制人外周血单个核细胞分泌γ-干扰素方面,冻存和新鲜的脐带间充质干细胞差异无显著性意义。经冻存复苏后的脐带间充质干细胞保持了间充质干细胞的基本形态,其表型满足间充质干细胞的基本要求,具有多向分化的潜能。
BACKGROUND： An effective freezing-thawing technique is crucial for the clinical application of human umbilical cord mesenchymal stem cells （UC-MSCs）. OBJECTIVE： To investigate biological characteristics of UC-MSCs after cryopreservation. METHODS： UC-MSCs were isolated from human umbilical cord and frozen in liquid nitrogen. The survival rate and the suppressive effect of γ-interferon （IFN-γ） of cryopreserved-thawed and fresh human UC-MSCs were compared. Furthermore, the multiple potentials and phenotype of UC-MSCs were estimated after cryopreservation. RESULTS AND CONCLUSION： There was no significant difference between cryopreserved-thawed and fresh human UC-MSCs on the survival rate and the suppressive effect of IFN-γ of peripheral blood mononuclear cells （PBMCs）. After cryopreservation, human UC-MSCs had the potential differentiation and the phenotype of mesenchymal stem cells.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第10期1729-1733,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
天津市科技创新专项资金项目(08FDZDSH02900)
课题名称:脐带间充质干细胞治疗产品的研制及细胞产品国家工程研究中心建设~~
