摘要
背景:动物实验中已多次验证,脐带干细胞移植可有效改善帕金森大鼠的旋转行为,且免疫排斥反应低。目的:以帕金森病统一评分量表作为观察指标,评估人脐带间充质干细胞移植对帕金森病的治疗效果。方法:纳入帕金森病患者15例,男8例,女7例,年龄52-76岁,帕金森病H&Y分级3-5级。在产妇知情同意情况下,通过医院伦理委员会批准,获取足月妊娠产妇的脐带,无菌收集脐带,培养脐带间充质干细胞。所有患者均住院治疗,自第2周起应用人脐带间充质干细胞进行颈动脉穿刺移植治疗。移植前及移植后1个月采用帕金森病综合评分量表对患者移植前后神经功能进行评定,分值越高表示神经功能缺损越严重。结果与结论:15例患者均进入结果分析。与移植前相比,移植后1个月患者帕金森病综合评分量表分值均明显降低(P<0.05),主要集中在对震颤、强直的改善,而运动迟缓、姿势不稳等临床症状无明显改善。患者均未出现移植物抗宿主病。提示脐带间充质干细胞移植治疗帕金森病效果显著,可明显改善患者的神经功能。
BACKGROUND：Animal experiments have repeatedly verified that umbilical cord stem celltransplantation can improve the rotational behavior of rats with Parkinson’s disease, with lower immune rejection response.OBJECTIVE：To evaluate the therapeutic effects of human umbilical cord mesenchymal stem celltransplantation in the treatment of Parkinson’s disease using the Unified Parkinson’s Disease Rating Scale. METHODS：Fifteen patients with Parkinson’s disease were enrol ed, including 8 males and 7 females, aged 52-76 years. Hoehn＆amp;Yahr staging was 3-5. After informed consent was obtained from puerperae and the procedure was approved by the hospital ethics committee, ful-term maternal umbilical cord was aseptical y col ected, to culture umbilical cord mesenchymal stem cells. Al patients were hospitalized, and treated with human umbilical cord mesenchymal stem celltransplantation via carotid artery puncture. Neurological function of patients was assessed using a comprehensive rating scale for Parkinson’s disease before and 1 month after cells transplantation. Higher score indicated more severe neurological deficits. RESULTS AND CONCLUSION：Fifteen patients entered the result analysis. Compared with before transplantation, 15 patients showed significantly lowered scores on the Unified Parkinson’s Disease Rating Scale＆amp;nbsp;at 1 month after transplantation （P〈0.05）. The improvement was mainly concentrated in the tremor and rigidity, but bradykinesia and unstable position were not improved. Graft versus host disease did not occur in al 15 cases. These findings indicate that umbilical cord blood mesenchymal stem celltransplantation for treating Parkinson’s disease has obvious curative effects and significantly improves neurological functions.
出处
《中国组织工程研究》
CAS
CSCD
2014年第6期932-937,共6页
Chinese Journal of Tissue Engineering Research
基金
河南省教育厅资助项目(2010B320013)~~
