摘要
目的:采用高效液相色谱法-蒸发光散射检测器(HPLC-ELSD)测定清咽胶囊中重楼皂苷Ⅰ和重楼皂苷Ⅱ的含量。方法:以乙腈-水(48∶52)为流动相,Hypersil C18柱(4.6 mm×250 mm,5μm)为固定相,流速1.0 mL.min-1,漂移管温度45℃,载气流量1.5L.min-1。结果:重楼皂苷Ⅰ的回收率97.27%,RSD为1.22%(n=6);重楼皂苷Ⅱ的回收率97.09%,RSD 1.40%(n=6)。结论:该方法简便、快速、重复性好,可用于清咽胶囊的质量控制。
Objective： To develop an HPLC-ELSD method for the content determination of Chonglou SaponinⅠand Chonglou Saponin Ⅱin Qingyan capsule.Method：The analysis was carried out on a Hypersil C18 analytical column（4.6 mm × 250 mm,5 μm） with the mobile phase consisting of acetonitrile-water（48∶ 52）.The detector drift tube temperature was set at 45 ℃ and the nitrogen flow rate was 1.5L.min-1.Result： The recoveries for Chonglou SaponinⅠand Chonglou Saponin Ⅱ were 97.27% and 97.09% with RSD 1.22% and 1.40%（n = 6）,respectively.Conclusion： The developed method is simple,rapid,replicable,and can be used for the quality control of Qingyan capsule.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第4期64-66,共3页
Chinese Journal of Experimental Traditional Medical Formulae
