摘要
目的：采用可见分光光度法测定重楼中总皂苷含量，确定最优显色方法。方法采用高氯酸反应使薯蓣皂苷元显色，对影响显色反应的主要因素进行了考察，于408nm波长处对重楼中总皂苷的含量进行测定。结果重楼总皂苷在3．74-26．18μg·mL^-1范围内线性关系良好（r=0．9998），平均回收率为101．1％，RSD为2．5％。结论：该方法简便、准确、灵敏，重复性良好，适用于重楼总皂苷的含量测定。
Objective ： A method for the determination of total contents of saponin to decide optimization method by ultraviolet spectrophotometry. Methods ： The color reaction betwen perchloric acid and diosgenin and the main factors af- fecting the reaction were studied. The diosgenin in paris polyphyUa was then determined by spectrophotometry at 408 nm. Results ： There was good linearity （ r = 0. 9998） during the range 3.74 - 26, 181μg · mL^-1. The average recovery was 101.1%, RSD was 2.5%, Conclusions ： The method is simple,sensitive and accurate,and it can be used as a quantita- tive analytical method for Paridis.
出处
《中华中医药学刊》
CAS
2007年第11期2420-2422,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
重楼
皂苷
分光光度法
Paridis
saportins
spectrophotometry UV
