摘要
以蛹虫草为材料,经过硫酸铵盐析、SephedexG!75柱层析和DEAE!52柱层析,得到纯化的超氧化物歧化酶(SOD),经聚丙烯酰胺凝胶电泳显示单一蛋白区带,此酶比活力为17855.73U/mg,纯化倍数为53.7,回收率为21.8%。该酶在40℃保温150min,活性不变;在pH值5.0～9.0有较好的稳定性。该酶每亚基含1原子铁,对H2O2和乙醇-氯仿溶液敏感。
Superoxide dismutase （SOD） from Chinese caterpillar fungus was separated and purified through ammonium sulfate precipitation and column chromatography with Sephadex G-75 gel filtration and DEAE-52 ion exchange. The purified enzyme showed a single band on PAGE. The specific activity was 17 855.73 U/mg, the SOD enzyme was 57.3-fold purified, 21.8 % of the SOD activity was recovered. The SOD from Chinese caterpillar fungus had good heat （40 ℃, 150 min） and acid-base stability in pH 5.0-9.0, in which the activity of SOD did not decline. Every subunit of this SOD contained one iron atom. The enzyme was sensitive to H2O2 and Tsuchihashi.
出处
《安徽农业科学》
CAS
北大核心
2005年第8期1388-1389,共2页
Journal of Anhui Agricultural Sciences
关键词
蛹虫草SOD
分离纯化
性质
Chinese caterpillar fungus
Superoxide dismutase
Purification
Character
