摘要
用人喉上皮细胞癌细胞系(HEP_2)的DNA转染人胚肺细胞(HEL),得到了5株基因转染细胞(GTC),命名为A5、B3、G8、D3和H3.这些GTC的染色体数在HEL的染色体数与两个亲代细胞(HEP-2和HEL)染色体的和数之间,感染人巨细胞病毒ADI69株后4d,D3比A5、B3、G8和H3有更多的荧光阳性细胞和更高的病毒滴度.在D3中HCMV复制随感染剂量的增大而增速.不同代数的D3对HCMV具有相似的敏感性,HCMV感染传至100代的D3,电镜证实仍可象原代D3复制大量的HCMV.永生性的D3可以用作分析调控HCMV复制的宿主细胞因子、分离培养HCMV等的工具.
Five gene transfected cells (GTC: A5,B3,D3,G8 and H3) were generated by DNA (from HEP-2) transfer (into HEL). Chromosome number of the transfectants fell between that of HEL and the combined chromosome number of the two cell lines. D3 showed more positive fluorescence cells than A5, B3, G8 and H3 by 4d after infection of HCMV. As HCMV infection dose was increased from 0.1 to 100 PFU/ ml, significant increase of viral production was observed. The similar susceptibility to HCMV was shown in different passages of D3. The immortal D3 may provide a tool to analyze host cell factors controlling the transcription and replication of HCMV.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第6期475-479,共5页
Acta Microbiologica Sinica
基金
江苏省卫生厅
计划生育委员会资助课题
