摘要
从中国牛外周血中分离淋巴细胞,提取基因组DNA.用所设计引物以聚合酶链式反应扩增出不致病的朊病毒蛋白(PrP^c)基因,并克隆到pGEM-TEasy Vector.序列分析表明所克隆的牛PrP^c的片段大小为795bp,包含了牛朊病毒基因的完整编码区序列.该基因无内含子,同国外报道的已知序列完全相同.
The total DNA was isolated from lymphocyte of peripheral blood of Chinese cattle. The PrPc gene was amplified by polymerase chain reaction, using two primers. It was then cloned into pGEM-T Easy Vector. The result of DNA sequencing was indicated that the 795bp amplified fragment contains the entire PrPc coding sequence, which has no intron and is same as the published gene sequence.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第6期417-421,共5页
Acta Microbiologica Sinica
关键词
牛朊病毒基因
DNA序列分析
基因克隆
Bovine prion protein gene, PrPC, DNA cloning,Sequence analysis
