摘要
根据已报道正常牛朊蛋白(PrPC)基因(PRNP)序列设计引物,采用PCR法扩增了6头荷斯坦奶牛的PRNP基因,将其克隆到T-Vector。序列测定及分析表明所克隆的奶牛PRNP基因片段为795bp,该基因内无内含子,包含了牛PRNP完整编码区序列,编码264个氨基酸的前体蛋白,推测其分子量约34ku。其中2头共同含有未曾报道的牛PRNP多态性位点M120I,无义突变G234A,但未引起酶切位点变异,未发现插入或缺失变异;与已报道牛PRNP序列(GenBank收录号为D10613)相比,两者核苷酸序列同源性为99%,其编码的氨基酸同源性为99%。
The total DNA was isolated from lymphocyte of peripheral blood of cattle. The PRNP gene was amplified by PCR method. The cloned PRNP gene was 795 nucleotides long and contains the entire PRNP coding sequence which has no intron and has 99% homology with the published gene sequence. And the deduced protein contain 264 amino acids, which had a calculated molecular weight (MW) of 34.0 ku. It is the first time the new alleles,Q264Q and G360A were found in the coding region of PRNP.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2004年第6期685-688,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金项目(30371062
30400325)
教育部博士点基金(20020019006)
