摘要
葱属（Ａｌｉｕｍ）为广义百合科（Ｌｉｌｉａｃｅａｅ）葱族（Ａｌｉｅａｅ）的一个重要类群。该属种类丰富、分布极广，关于它的系统发育和进化问题，目前尚存在不少分歧［１］。我国有葱属１１０种（含变种和引进外来种），主要分布于东北、华北、西北和西南地区。我们...
The molecular phylogeny of the genus Allium which includes eighteen species selected from nine sections was investigated through PCR RFLP analysis of two chloroplast DNA fragments, including trnK gene (approximately 2520 bp) and rpL 16 gene (approximately 1230 bp). Digestion of these two fragments by 26 restriction endonucleases yielded 303 polymorphic recognition sites, of which 163 were informative sites. The restriction site data matrix were analyzed following the parsimonious Wagner and parsimonious Dollo principle of PAUP(version 3.1.1). Topologically, the most parsimonious Wagner tree constructed by branch and bound and heuristic search was similar to the most parsimonious Dollo tree. All the taxa of Allium form a monophyletic group, and five sections based on morphological characters were supported strongly by this result. Sect. Auguinum is closely related to Sect. Bromatorrhiza, Sect. Molium is closely related to Sect. Caloscordum. Their reliability was farther confirmed by the bootstrap test very well. In morphology, A. pallasii is closely related to A. caeruleum and belongs to Sect. Haplostemen, A. cepa is closely related to A. galanthum and belongs to Sect. Cepa. But evidence from cladistics of parsimonious tree based on 163 informative sites of PCR RFLPs showed that they are neither confined to a monophyletic group nor to a natural taxon.
基金
国家自然科学基金
中国科学院植物研究所系统与进化植物学开放研究实验室资助
关键词
葱属
分子系统发育
RFLP
百合科
Allium , Molecular phylogeny, RFLP, trnK gene, rpL 16 gene
