摘要
用脂质体包裹Ｅｉｍｅｒｉａｍａｘｉｍａ孢子化卵囊抗原给鸡口服免疫，以１０３个孢子化Ｅ．ｍａｘｉｍａ卵囊／只的剂量攻毒，可使鸡获得４６％的保护力。ＥＬＩＳＡ检测结果表明，鸡体在免疫后产生了特异性抗体。ＳＤＳ－ＰＡＧＥ结果表明，所提取Ｅ．ｍａｘｉｍａ孢子化卵囊可溶性抗原至少有１６条带，其中８条主带分子量分别为：＞９７．４ｋＤ、４７．９ｋＤ、４２．７ｋＤ、３５．５ｋＤ、２９．５ｋＤ、２３．４ｋＤ、１３．５ｋＤ、１０．５ｋＤ。用Ｗｅｓｔｅｒｎ－ｂｌｏｔｉｎｇ分析结果表明，口服活卵囊初次免疫１４ｄ后的鸡血清，可识别出４条带，其分子量分别为：＞９７．４ｋＤ、６０．３ｋＤ、４７．９ｋＤ、４２．７ｋＤ。
Protection percentage was 46% after chickens immunized orally with lipsome entrapped antigens of Eimeria maxima sporulated oocysts were challenged with 1000 sporulated E.maxima oocysts per chicken.It was showed that the chickens immunized with lipsome entrapped antigens produced specific IgG by ELISA.The predominant proteins of E.maxima sporulated oocysts separated by SDS PAGE were 47 9kD,42 7kD,35 5kD,29 5kD,23 4kD,13 5kD,10 5kD and one band of >97 4 kD,respectively.When probed with sera obtained on day 14 post primary infection with E.maxima oocysts by western blotting,the major antigens of E.maxima sporulated oocysts recognized by anti E.maxima antibodies were 60 3kD,47 9kD,42 7kD and one of >97 4kD,respectively.
出处
《寄生虫与医学昆虫学报》
CAS
1998年第4期205-210,共6页
Acta Parasitologica et Medica Entomologica Sinica
