摘要
采用单卵囊感染技术，从鸡球虫混合种中分离出一个纯种球虫，经鉴定为巨型艾美耳球虫（ＥｉｍｅｒｉａＭａｘｉｍａＴｙｚｚｅｒ，１９２９）。该种球虫卵囊为卵圆形，测２４０个的大小范围为２１．２５～３６．２５×１７．５～３０．０μｍ，平均为２８．８１±３．０７×２２．９８±２．７７μｍ，形状指数１．２５。卵囊内有一颗折光性极粒，位于窄端，无外残体。测１２０个孢子囊的大小范围为１５．５～１８．５×７．５～１０．０μｍ，平均为１７．３５±０．６６×８．７４±０．５１μｍ。最短孢子化时间为３０小时，潜伏期１４２小时。用此卵囊对鸡作致病性试验，挑选６０羽１３日龄伊莎（ＩＳＡ）公鸡分为１个不感染组和４个感染组，每羽口服感染１万、５万、１０万或２０万个孢子化卵囊。结果所有感染组与不感染组的平均增重差异达到显著（Ｐ＜０．０５）或极显著（Ｐ＜０．０１）水平，整个试验仅在２０万个的感染组出现１６．７％的死亡率，各感染组的肠道病变记分分别为１．３３、１．８３、２．４２和３．０８。
Using single oocyst infected techniques. we have gained a pure species from mixed species of chicken coccidia and identified Eimeria maxima (Tyzzer, 1929). The oocyst shape is ovoidal. The average length and width of 240 oocysts examined is 28.81±3.07 × 22.98 ±2.77 μm. The shape index is 1.25. The oocyst has a polar granule and no extra-residual body. The mean length and width of 120 sporocysts examined is 17.35±0.66×8.74±0.51 μm.The minimum sporulatic time and prepatent period are 30 and 142 h. A total of 60 13-day-old ISA birds were divided into 5 groups of 12 chickens each. Group 1 was noninfected controls. Group 2 to 5 received orally 1 × 104, 5 × 104, 10 ×104 and 20 ×104 sporulatic oocysts, respectively. The results showed that the difference of mean body weight gain was significat (P<0.05) or very significant (P<0.01) between infected groups and noninfected group. The mortality of the chickens. which was only infected with 20 ×104 oocysts, was 16.67%. The intotine lesion scoring of 4 infected groups were 1 .33, 1 .83, 2.42 and 3 .08, respectively.
出处
《中国兽医寄生虫病》
CAS
1995年第4期12-14,共3页
Chinese Journal of Veterinary Parasitology
基金
农业部"八五"项目
