摘要
研究了嗜碱芽孢杆菌（ａｌｋａｌｏｐｈｉｌｉｃＢａｃｌｕｓｓｐ．）ＮＴＴ３３发酵产生胞外碱性β－甘露聚糖酶的条件，其最佳碳源为１％槐豆角，最佳氮源为１％蛋白胨＋０．２％酵母膏，发酵培养３６ｈ产酶量最高（达６１．３υ／ｍＬ）。甘油，葡萄糖，甘露糖等对产酶有强的阻遏作用。该菌株经紫外诱变处理后，采用透明圈法初步筛选出在含葡萄糖和不含葡萄糖的槐豆胶培养基上同时产生透明圈的菌株，进一步测定其产酶进程曲线，最后筛选到一株（ＮＴＴ３３－ｒ６）部分消除葡萄糖代谢阻遏高产β－甘露聚糖酶的菌株，其酶活力比出发菌株提高５０％，，达到９６．３Ｕ／ｍｌ；。
The optimal conditions for production of extracellular alkaline β mannanase by alkalophilic Bacillus sp. NTT33 were reported. It was found that 1% locust bean gum,1% peptone+0.2% yeast extract were the best carbon andnitrogen source for the enzyme production, respectively. The optimal time of enzyme production was 36h (up to 61.3L/ml). The enzyme production was strongly inhibited by glycerol, glucose, mannose.After treatment with UV light, strains were selected by comparing the diamter of clearace zones produced by the survivors in BM and GB. Time course of enzyme production which was determined in BM ang GB liquid media, respectively. Strain NTT33-y6 which produced high β mannanase activity was selected. The catabolic repression caused by glucose was partly eliminated from the strain. β-mannanase activity was 50% higher than that produced by wild type strain NTT33 and the enzyme yield of the strain could reach to 96.3U/ml.
出处
《氨基酸和生物资源》
CAS
1998年第4期14-18,共5页
Amino Acids & Biotic Resources
关键词
嗜碱芽孢杆菌
甘露聚糖酶
发酵
诱变育种
alkalophilic bacteria alkalin β mannanase fermentation conditions mutation
