摘要
为探索同步测定大鼠成骨细胞膜Ｃａ２＋－ＡＴＰ酶和Ｎａ＋、Ｋ＋－ＡＴＰ酶活性的方法。采用孔雀绿比色法测定磷以反映酶活性。结果显示：Ｃａ２＋浓度以及成骨细胞传代培养的代数是影响酶活性的重要因素。Ｃａ２＋有助于提高ＡＴＰａｓｅ活性。Ｃａ２＋浓度为４０μｍｏｌ／Ｌ时，ＡＴＰａｓｅ活性较高。细胞传代越多，膜Ｃａ２＋－ＡＴＰａｓｅ活性越低。成骨细胞膜Ｃａ２＋－ＡＴＰａｓｅ活性低于Ｎａ＋、Ｋ＋－ＡＴＰａｓｅ。结论：孔雀绿比色法同步测定大鼠成骨细胞膜Ｃａ２＋－ＡＴＰ酶和Ｎａ＋、Ｋ＋－ＡＴＰ酶活性，方法简便、灵敏、可靠。
The aim of this study was to find a method to measure simultaneously the activities of Ca 2+ ATPase and Na +, K + ATPase of rat osteoblast membrane. In this test, Ca 2+ ATPase and Na +, K + ATPase released inorganic phosphate (Pi) from ATP, and Pi was determined by colorimetric method using malachite green dye to show ATPase activities. Ca 2+ concentration and osteoblast culture generation were important factors affecting ATPase activities. Ca 2+ could elevate membrane ATPase activity. When Ca 2+ concentration reached 40 μmol/L, ATPase activities were improved. Ca 2+ ATPase activity decreased with the increase of osteoblast culture generation. Ca 2+ ATPase activity was lower than Na +, K + ATPase activity in osteoblast membrane. This method is simple, sensitive and reliable.
出处
《华西医科大学学报》
CSCD
1998年第4期427-430,共4页
Journal of West China University of Medical Sciences
基金
国家自然科学基金
