摘要
目的 研究汉防已甲素对急性缺血性肾衰的保护作用。方法 术前 ３ｄ开始每天 １次及术前 １ｈ ｉｇ汉防已甲素 ２０ｍｇ／ｋｇ、４０ ｍｇ／ｋｇ。然后制作大鼠肾脏缺血再灌注致急性缺血性肾衰模型，测定肾组织中 ＭＤＡ、ＳＯＤ、Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋－ＡＴＰ酶含量及切片观察肾脏形态。结果 缺血6０ｍｉｎ再灌注２０ｍｉｎ后肾组织中 ＭＤＡ明显升高，ＳＯＤ、Ｎａ＋、Ｋ＋－ＡＴＰ酶和 Ｃａ２＋－ＡＴＰ酶均显著降低。用药组 ＭＤＡ显著下降，ＳＯＤ和 ＡＴＰ酶均明显升高。病理结果提示再灌注组肾小管上皮明显变性，而用药组损害明显减轻。结论 汉防已甲素对急性缺血性肾衰有一定的保护作用。
Aim: To study the protective effects of tetrandine(Tet) on acute ischemic renal failure in rats. Methods Tet 20mg/kg, 40mg/kg once daily, for three days and one hour before operation was administered orally to rats. The models of renal ischemia and reperfusion in rats were built. The malondialdehyde(MDA), superoxide dismutase(SOD), Na+ , K+-ATP ase and Ca2+-ATPase of renal tissue were determined and the histopathological structure of kidneys was obseved. Re-sults In the reperfusion group, the level of MDA was increased and the levels of SOD, Na+ K+-ATP ase and Ca2 +-ATPase were decreased significantly. In the Tet group the level of MDA was decreased and the levels of SOD, Na + , K +-ATPas and Ca2 ATP ase were in-creased significantly. The wide denaturation of epithelial cell of uriniferous tubules was observed by light microscope in the reperfusion group-but little in the Tet group. Conclusion Tet has protective effects on acute ischemic renal failure in rats.
关键词
汉防已甲素
大鼠
急性缺血性肾衰
保护作用
tetrandrine
acute isehemic renal failure
malondialdehyde
superoxide dismutase
ATPase
