摘要
以沙田柚的实生苗不同外植体为对象,进行了沙田柚遗传转化再生体系建立研究。研究结果表明:以实生苗上胚轴作为外植体,以MS无机盐+100mg/L肌醇+0.2mg/L维生素B1+1mg/L维生素B6+1mg/L烟酸+3%蔗糖+0.8%琼脂(pH5.8)+5mg/L6-BA为不定芽诱导培养基,暗培养7天后,转移至光/暗周期16/8h的条件下培养,不定芽的分化率高达91.1%;不定芽在1/2MS基本培养基+3%蔗糖+0.7%琼脂+1.5mg/LIBA+0.2%活性炭的生根培养基,生根率高达80%;以卡那霉素作选择剂时,选择浓度为50mg/L。
Transgenic plants appear to be a new way to citrus improvement and a powerful tool for studying gene functions. The establishment of an efficient in vitro plant regeneration system suitable for genetic transformation is the first step in this approach. Different explants of seedlings were grownin vitro and a efficient regeneration system was established for Citrus grandis Osbeck cv. Shatianyou. The results were obtained as follow. When the epicotyl pieces were explants were cultured on the adventitious shoot regeneration medium （MS salts ＋100 mg/L inositol ＋0.2 mg/LV-B1＋1 mg/LV-B6＋1 mg/L niacin ＋ 3% sucrose ＋ 5 mg/L6- BA ＋ 0.8% agar,pH5.8） and incubated in darkness for 7 days,then in the condition of 16/8 h light/ darkness cycle,adventitious shoot regenerated from 91.1% of the epicotyl explants. When the adventitious shoots were cultured on the medium （1/2MS＋1.5 mg/L IBA ＋3%sucrose ＋0.7%agar,pH5.8） , adventitious root regenerated from 80% of the adventitious shoot after grown in vitro for 40d. As a selectable agent, concentration of Kanamycin is 50 mg/L for epicotyl pieces.
出处
《中国农学通报》
CSCD
北大核心
2010年第7期177-180,共4页
Chinese Agricultural Science Bulletin
基金
国家转基因植物研究与产业化开发专项"柑橘规模化转基因技术研究及优异种质的创新"(JY04-B-02)
关键词
沙田柚
遗传转化
离体再生
Citrus grandis Osbeck cv. Shatianyou
transformation
in vitro regeneration
