摘要
以Thatcher及23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系为材料,开展了MITE扩增体系的建立研究,获得了适合小麦MITE分子标记的扩增体系及不同引物的退火温度,所用56对引物有27对获得稳定扩增。
Thatcher and 23 of wheat leaf rust resistance near isogenic lines in Thatcher background were chosen as materials in this experiment. Research on the establishment of MITE amplification system was carried out. 27 of the 56 primer pairs were amplified stably,reaction system and different annealing temperature of primers were confirmed for amplification of MITE（ Miniature inverted repeat transposable elements）.
出处
《华北农学报》
CSCD
北大核心
2014年第2期110-115,共6页
Acta Agriculturae Boreali-Sinica
基金
国家重点基础研究发展计划"973计划"项目(2013CB127700)
国家自然科学基金项目(30771391)
