摘要
目的:观察电针治疗神经病理性疼痛大鼠对脊髓背角胶质纤维酸性蛋白(GFAP)的活化以及促炎性细胞因子TNF-α(肿瘤坏死因子-α)和IL-1β(白介素-1β)表达的影响。方法:72只SD大鼠随机分为3组:假手术组(仅分离坐骨神经)、模型组[结扎坐骨神经造成慢性压迫性损伤(CCI)]和电针组(手术后连续6 d取"足三里""环跳"穴给予电针治疗)。手术前1天和手术后第7天测定各组动物的机械性痛阈和热痛阈。采用免疫组化的方法观察大鼠脊髓L4-L5GFAP的变化,并用荧光定量多聚酶链反应技术分别检测TNF-α mRNA和IL-1βmRNA表达变化。结果:CCI可导致大鼠机械性痛阈和热痛阈明显降低,显著激活损伤侧脊髓GFAP,脊髓中TNF-α和IL-1β mRNA的表达明显增多(均P<0.05)。给予电针治疗后能明显改善CCI大鼠痛敏状态,并显著降低损伤侧脊髓GFAP活性和TNF-α和IL-1β mRNA的表达(均P<0.05)。结论:电针"足三里""环跳"可明显减轻CCI大鼠的疼痛反应,其作用与其降低脊髓GFAP、TNF-α mRNA和IL-1β mRNA的表达有关。
Objective To observe the effect of electroacupuncture （EA） on the expression of spinal glial fibrillary acidic protein （GFAP）. tumor necrosis factor-α（TNF-α） and interleukin-1β（IL-1β） in chronic constriction injury （CCI） rats. Methods Seventy-two SD rats were randomized into sham operation （sham）, CCI （model） and EA groups （n = 24/group）. The mechanical and thermal pain thresholds were measured by using Von Frey filaments and radiant-heat irridiation separately. The immunoactivity of GFAP of spinal dorsal horn （L4 - L5 ） was assessed by immunohistochemistry , and the expression of spinal TNF-α mRNA and IL-1β mRNA was detected by real time-PCR. Results Compared with pre-CCI and sham group, both mechanical and thermal pain thresholds decreased considerably in rats with CCI （ P〈0.05）, and in comparison with model group, those of EA group increased markedly （ P〈0.05）. Compared with sham group, GFAP immunoactivity （mainly in the lamina Ⅰ-Ⅱ of the spinal dorsal horn）, TNF-α mRNA and IL-1β mRNA expression in the ipsilateral spinal cord on the COl side in model group increased considerably （ P〈0.05）, while compared with model group, the expression of GFAP, TNF-α mRNA and IL-1β mRNA in EA group was down-regulated remarkably （P〈0.05）. Conclusion EA can effectively suppress CCI-induced up-regulation of expression of spinal GFAP, TNF-α mRNA and IL-1β mRNA, which may contribute to its effect in reducing mechanical allodynia and thermal hyperalgesia in neuropathic pain rats.
出处
《针刺研究》
CAS
CSCD
北大核心
2010年第1期12-16,共5页
Acupuncture Research
基金
国家自然科学基金(No.30801072)
山东省自然科学基金(No.Q2007C14)资助项目
