摘要
目的:探讨青藤碱、环孢素及两者联合应用对大鼠肝移植模型排斥反应的影响。方法:正常组:8只Wistar,为空白对照。建立SD-WistarOLT模型,分为4组,每组8只SD-Wistar OLT大鼠。对照组、CsA组、SIN组、亚剂量CsA+SIN组。检测血浆IL-2、IL-10含量,肝内TGF-β1表达,肾脏TGF-β1mRNA表达,肝脏内淋巴细胞的凋亡,肝脏的病理损害。结果:SIN+CsA组抑制IL-2及促进TGF-β表达弱于CsA组,促进IL-10分泌强于CsA组,同时促进了肝内淋巴细胞的凋亡,减轻了肝脏的病理损害。结论:SIN能够减轻肝脏移植后的肝脏损害;与CsA联合作用减少CsA的用量,防止肾纤维化的发生。
Objective： To compare the effect of CsA, SIN and their combination on the rejection reaction after the orthotropic liver transplantation in rats. Methods： The blank group was eight Wister rats. We established the stable orthotropic liver transplantation （ OLT ） model. The rat models were randomly divided into four groups and every group was eight SD-Wister rats, Control group, CsA treated group, SIN treated group, SIN and half-value dose CsA treated group. Enzyme-linked immunosorbent assays was carried out to detect the level of IL-2, IL-10. The expression of TGF-β protein in liver and TGF-β mRNA in kidney used immunohistochemisty staining and RT-PCR, respectivly. The lymphocyte apoptotic index in header district of liver was detected using the TUNEL.The liver pathological lesion was scored with Williams＇s system after HIM staining. Results： The level of IL-2 and expression of TGF- β 1 in the SIN＋CsA-treated group was lower than CsA-treated group, but the level of IL-10 was stronger than the CsA. The lymphocyte apoptotic index in header district of SIN＋CsA-treated group was the most obvious and the damage of the transplantation liver was the lightness than other groups.Conclusion： SIN could alleviate the pathological damage after OLT in rats. SIN and CsA combination could reduce the dosage of CsA, and Prevented of renal fibrosis.
出处
《辽宁中医药大学学报》
CAS
2010年第4期112-114,共3页
Journal of Liaoning University of Traditional Chinese Medicine
关键词
肝移植
青藤碱
环孢素A
急性排斥反应
肾纤维化
liver transplantation
sinomenine
eyclosporin A
acute rejection
renal fibrosis
