摘要
目的：建立测定不同厂家牡丹皮药材中丹皮酚含量的高效液相色谱法。方法：以Hypersil C18柱（5μm，4．6mm×250mm）为色谱柱，流动相为甲醇-水（45：55），流速为1．0mL/min，检测波长为274nm。结果：丹皮酚线性范围为0．026416～0．079248p．g，r2=0．9999；平均回收率为98．325％，RSD为1．22％。结论：采用HPLC以丹皮酚含量为指标比较牡丹皮饮片质量，该法简便易行。
Objective：To establish HPLC method for the determination of paeonol in Cortex Moutan from different factories. Methods： The Hypersil Cl8 （4.6mm × 250mm, 5 μm） was used with methanol： water （45 ： 55 ） as mobile phase. The flow rate was 1.0 mL · min - 1. The deteeting wavelength was 274 nm. Results： The linear of paeonol was in the range of 0. 026416 ～ 0.079248μg, r2 = 0. 9999. The average reeovery was 98. 325% and RSD was 1.22%. Conclusion：The method was simple, reliable, aeeurate and suitable for the quality control of paeonol in Cortex Moutan.
出处
《黑龙江医药》
CAS
2010年第2期151-153,共3页
Heilongjiang Medicine journal
