摘要
目的：建立同时测定复方黄白胶囊中绿原酸、栀子苷和芍药苷3种成分含量的方法。方法：采用高效液相色谱法。色谱柱为ZORBAXEclipse SB—C18（250mm×4．6mm，5μm），流动相为乙腈-2％。磷酸水溶液（梯度洗脱），流速为1．0ml／min，柱温为30℃，绿原酸、栀子苷和芍药苷的检测波长分别为327、240和232nm。结果：绿原酸、栀子苷和芍药苷的进样量分别在0．02～0．19、0．09～0．84和0．11～1．01μg范围内与各自峰面积积分值呈良好线性关系（r分别为0．9997、O．9998、0．9999）；精密度、稳定性、重复性试验的RsD〈3％；平均加样回收率分别为99．6％、101．0％和100．2％，RSD分别为1．3％、0．7％和1．4％（”均为6）。结论：所建方法可用于复方黄白胶囊中3种成分的含量测定。
OBJECTIVE： To establish a method for simultaneous determination of chlorogenic acid, geniposide and paeoniflorin in Compound huangbai capsules. METHODS： HPLC method was adopted. The determination was performed on ZORBAX Eclipse SB-Cs （250 mm× 4.6 mm, 5 μm） column with acetonitrile-0.2%c phosphoric acid as mobile phase （gradient elution） at the flow rate of 1.0 ml/min. The column temperature was at 30 ℃. The detection wavelengths were set at 327, 240 and 232 nm for chlorogenic acid, geniposide and paeoniflorin, respectively. RESULTS： The linear ranges of chlorogenic acid, geniposide and paeoniflorin were 0.02-0.19 μg（r=0.999 7）, 0.09-0.84 μg（r=0.999 8） and 0.11-1.01 μg（r=0.999 9）, respectively. RSDs of precision, stability and reproducibility tests were all lower than 3%. The average recoveries of the 3 components were 99.6% （RSD=1.3% ,n=6）, 101.0% （RSD=0.7% ,n=6） and 100.2%（RSD=1.4% ,n=6）, respectively. CONCLUSIONS： The established method can be applied for the simultaneous determination of 3 components in Compound huangbai capsules.
出处
《中国药房》
CAS
CSCD
2014年第7期641-643,共3页
China Pharmacy
基金
黑龙江省教育厅科学技术研究项目(No.12521547)
佳木斯大学科学技术面上项目(No.S2011-032)
关键词
复方黄白胶囊
绿原酸
栀子苷
芍药苷
高效液相色谱法
多波长
含量测定
Compound huangbai capsules
Chlorogenic acid
Geniposide
Paeoniflorin
HPLC
Multi-wavelength
Content determination
