摘要
研究了叶酸（Folic Acid，FA）在多壁碳纳米管修饰玻碳电极（MWCNT／GCE）上的电化学行为．实验结果表明，FA在GCE上的直接电化学氧化十分迟缓，无氧化峰出现，而在MWCNT／GCE上0．681V处出现一个不可逆氧化峰，表明MWCNT／GCE对FA具有良好的电催化作用．测定了FA在MWCNT／GCE上的电催化过程动力学参数，电子转移系数a为0．80，扩散系数D为6．217×10^-5cm^2／s，电极反应速率常数k1为2．15×10^-5cm^2／s．在8×10^6～2×10^-4mol／L浓度范围内，FA峰电流与其浓度呈良好的线性关系，线性方程为Ips（uA）=70．46c＋0．046，r=0．9995，检出限为3×10^-6umol／L,对市售药品进行定量测试，所测样品RSD为1．8％～4．8％，加标回收率为95．5％～102．1％．可用于FA电化学定量测定．
Electrochemical behavior of folic acid （FA） at a multi-walled carbon nanotube modified glassy carbon electrode （MWCNT/GCE） has been investigated. The experimental results demonstrated that the direct electrochemical oxidation of FA were sluggish on GCE, no oxidation peaks appeared. However, on MWCNT/GCE it appeared an irreversible oxidation peak at 0. 681 V, which indicates that the modified electrode exhibits a good promoting effect on the electrochemical reaction of FA. The electrochemical kinetics parameter has been determined： the electron transfer coefficient a was 0. 80, the diffusion coefficient D was 6. 217 ×10^-5cm^2/s and the electrode reaction rate constant kf was 2.15×10^-2cm/s. It is found there is a good linear relationship between the anodic peak current and the FA concentration from 8×10^-6 to 2×10^-4 mol/L. The regression equation is Ips （uA）：70. 46c＋0. 046, r= 0. 999 5. The detection limit of the method is 3 ×10^-6 mol/L. This method was applied to the determination of FA in drug tablets, RSD are from 1.8% to 4.8%. The recoveries are from 95.5% to 102.1%, which can be used in FA electrochemical quantity determination.
出处
《宁夏大学学报（自然科学版）》
CAS
北大核心
2010年第1期70-73,共4页
Journal of Ningxia University(Natural Science Edition)
基金
宁夏高等学校科学研究基金资助项目(200623)
关键词
叶酸
碳纳米管修饰电极
电催化氧化
电分析方法
folic acid
carbon nanotubes modified glassy carbon electrode
electrochemical kinetic
electroanalytical method
