摘要
采用1，2-萘醌-4-磺酸钠体系分光光度法测定磺胺甲嗯唑（SMZ）。磺胺甲嗯唑与1，2-萘醌-4-磺酸钠在pH=10．0的缓冲溶液中发生亲核取代反应生成橙红色的产物，组成比为1：1，最大吸收波长λ=476nm；表观摩尔吸光系数ε=6．44×10^3 L／（mol·cm）；SMZ浓度在0．2～60mg／L范围内呈良好的线性关系；线性回归方程为A=0．02452＋0．02301C（mg／L），线性相关系数r=0．9991；检出限为0.08mg／L；RSD为0．25％（20mg／L，n=11）；平均回收率为98．7％以上。优化了对磺胺甲嗯唑的测定条件。初步探讨了反应机理，应用拟定的方法测定磺胺甲嗯唑的含量，与药典法相比，结果满意。
A simple and sensitive spectrophtometric method is described for the determination of sulfamethoxazole. The method is based on a nueleophilic substitution reaction to measure the salmon pink compound produced by the reaction of sulfamethoxazole with sodium 1,2-naphthoquinone- 4-sulfonate in pH 10.0 buffer solution. The stoichiometric ratio of the compound is 1 ： 1, and its maximum absorption wavelength is at 476 nm,ε=6.44 × 10^3 L/（mol· cm）. The Beer＇s law is obeyed in the range of 0.2 -60 mg/L of sulfamethoxazole. The linear regression equation is A =0.02452 ＋ 0. 02301C （mg/L）, with a linear correlation coefficient of 0.9991. The detection limit is 0.08 mg/L, RSD is 0.3% （20 mg/L, n = 11 ）, and average recovery is over 98.7%. The reaction mechanism is studied intensively. This proposed method has been successfully applied to the determination of sulfamethoxazole with satisfactory results.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第1期83-86,共4页
Chinese Journal of Analytical Chemistry
