摘要
目的 探讨邻苯二甲酸正丁酯(DBP)对急性白血病患者骨髓原代白血病细胞增殖与凋亡的影响.方法应用体外液体悬浮培养观察细胞增殖;应用DNA片段凝胶电泳方法观察细胞凋亡;组间差异用t检验.结果DBP50μg/ml和200μg/ml处理24小时,对急性白血病原代细胞增殖抑制率分别为24.24%和36.33%,来自急性髓系白血病的原代细胞较急性淋巴细胞性白血病原代细胞对DBP抑制作用更敏感.DBP诱导白血病原代细胞凋亡,在DNA片段凝胶电泳时显示典型的细胞凋亡表现.结论 DBP可能通过抑制急性白血病细胞增殖和诱导其凋亡来发挥净化白血病骨髓的作用.
PURPOSE To sutdy the effects of dibutyl pathalate (DBP) on the proliferation and apoptosis of leukemic cells from patients suffering from acute leukemia. METHODS The cell culture in vitro was used to study the effect of DBP on the proliferation of leukemic cells, while agrose gel electrophoresis of DNA fragments was used to study the effect of DBP on apoptosis of leukemic cells. RESULTS DBP could inhibit the proliferation of leukemic cells got from patients suffering from acute leukemia in vitro. The percentage of inhibition were 24.24% and 36.33% respectively when leukemic cells were exposed to 50μg/ml and 200μg/ml DBP for 24 hours. Leukemic cells from acute myelogenous leukemia were more susceptive to DBP than those from acute lymphocytic leukemia. DBP also induced the leukemic cells from patients to die via apoptosis and showed typical 'ladder' of DNA fragments for apoptotic cells in agrose gel electrophoresis. CONCLUSION DBP could clear the leukemic cells in bone marrows of patients suffering from acute leukemia by suppressing the growth of leukemic cells and inducing them to die via apoptosis.
出处
《中国癌症杂志》
CAS
CSCD
1998年第4期289-291,共3页
China Oncology
关键词
急性白血病
细胞增殖
细胞凋亡
DBP
Dibutyl pathalate Acute leukemia Cell proliferation Cell apoptosis Puring in vitro
