摘要
目的建立蒙药玉簪花中山奈酚-7-O-β-D-葡萄糖苷的含量测定方法。方法采用HPLC法,色谱柱为Phenomenex Luna C18(250mm×4.6mm,5μm),以体积分数为0.5%的磷酸水溶液-甲醇为流动相,梯度洗脱,检测波长为364nm。结果山奈酚-7-O-β-D-葡萄糖苷质量在0.113～2.260μg内呈良好线性关系,相关系数为1,平均回收率为101.1%(RSD=2.58%,n=6)。结论玉簪花中山奈酚-7-O-β-D-葡萄糖苷的含量为0.2mg/g(0.02%),该方法简便、准确、重现性好,可用于蒙药玉簪花中山奈酚-7-O-β-D-葡萄糖苷的含量测定。
Objective To establish a HPLC method for the determination of kaempferol-7-O-β-D-glucopyranoside in Hosta ventricosa stearn. Methods HPLC analysis was carried out on a Phenomenex Luna C18 （250 mm×4.6 mm, 5 μm） column, gradient elution with the mobile phase of 0.5 % phosphate acid in water-methanol. The detective wavelength was set at 364 nm. Results calibration curve was linear within the range of 0.113- 2.260 μg for kaempferol-7-O-β-D-glueopyranoside, coefficient correlation being 1. The average recovery was 101.1% （RSD= 2.58 %, n=6）. Conclusion The content of kaempferol-7-O-β-D-lueopyranoside in Hosta ventricosa steam is 0.2 mg/g（0.02 %） on average. This method is simple, aeeurate and practical for the determination of kaempferol--7-O-β-D-glucopyranoside.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2010年第2期192-194,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
