摘要
本文建立了茶叶中常用的30种农药的快速分析方法。以0.1%乙酸的乙腈(V/V)作为溶剂,Agilent SampliQ提取试剂对样品进行1min提取后,用C18和Agilent SampliQ净化试剂净化,用10ml0.1%乙酸的乙腈与甲苯(体积比3:1)洗脱,气相色谱-质谱(GC-MS)检测。30种农药的检测限(LOD)为0.03μg/kg(氟草胺)～13μg/kg(苯胺灵)。采用内标法定量,添加回收试验结果表明,30种农药的平均回收率为74%～108%,相对标准偏差为0%～10.38%。该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。
A method for the fast determination of 30 pesticides in tea was developed. Tea samples were extracted with acetonitrile （MeCN） containing 0.1% acetic acid （Hac） as solvent, then added Agilent SampliQ extraction reagent for 1 minute and clean-up by C18 and Agilent SampliQ clean-up reagent, eluted with 10mL acetonitrile-toluene （3： 1, v/v） and determined by gas chromatography- mass spectrometry （GC-MS）. The limits of detection （LODs） ranged from 0.03 μ g/kg （benfluralin） to 13μ g/kg （profam） and quantified by internal standard method. The recoveries of those pesticides were 74% - 108%, and the relative standard deviations were 0% 10.38 %. The sensitivity, Accuracy and precision of the method all satisfy the requirement of pesticide residue analysed.
出处
《中国食物与营养》
2010年第2期57-60,共4页
Food and Nutrition in China
基金
国家质检总局科研课题(No.2009IK285)
关键词
QUECHERS
气相色谱-质谱
茶叶
农药
多残留检测
QuEChERS
Gas Chromatography-Mass Spectrometry（GC-MS）
Tea
Pesticides
Multiresidue Determination
