摘要
用明胶将辣根过氧化物酶(HRP)固定于多壁碳纳米管(MWNT)和茜素红(AR)修饰的玻碳(GC)电极上,制成HRP生物传感器(HRP/AR/MWNT/GC),然后在3%戊二醛(GA)中进行交联改性,以克服明胶膜易溶胀的缺点,并提高膜的稳定性.同时详细探讨了该传感器对H2O2的响应性能,并优化了实验条件.结果表明,该传感器对H2O2的线性响应范围为5.0×10-6～1.0×10-3mol/L,线性相关系数为0.9932,检出限为1.0×10-7mol/L,且放于4℃环境30d后,峰电流值约为原来的72.1%.该传感器响应快速,灵敏度高,且具有良好的重现性、稳定性及较长的使用寿命,具有潜在的应用价值.
A novel hydrogen peroxide biosensor was obtained by successfully immobilizing horseradish peroxidase（HRP） on a glassy carbon（GC） electrode,which was modified with multi-walled carbon nanotubes（MWNT） and alizarin red（AR）.Then the biosensor was immerged in 3% glutaraldehyde（GA） in order to overcome the swelling and improve the stability of the gelatin membrane.The experimental condition was optimized,and the electrochemistry behavior of the HRP/AR/MWNT/GC biosensor in PBS（pH 7.0） and H2O2 was discussed.The biosensor displayed rapid response and expanded linear response range from 5.0×10^-6 to 1.0×10^-3 mol/L（R=0.9932） with detection limit of 1.0×10^-7 mol/L in PBS（pH 7.0）,as well as acceptable preparation reproducibility and excellent stability.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2010年第3期257-262,共6页
Acta Chimica Sinica
关键词
多壁碳纳米管
茜素红
明胶
辣根过氧化物酶
戊二醛
multi-walled carbon nanotube
alizarin red
gelatin
horseradish peroxidase
glutaraldehyde
