摘要
提出了一种以电沉积铁氰酸镍(NiHCF)无机膜为介体的胆碱传感器的制备方法。该传感器以壳聚糖为酶固定基质,用戊二醛作交联剂分别固定辣根过氧化物酶和胆碱氧化酶。该传感器在pH6.8,外加电压-0.1V条件下,对2.0×10-5～1.0×10-3mol/L的胆碱呈良好的线性响应,检出限为5.0×10-6mol/L。传感器有良好的选择性和稳定性,使用1个月后,仍能保持其初始活性的75%。
Acholine biosensor based on the chitosan filmimmobilizing horseradish peroxidase and choline oxiˉdase has been developed.Nickel hexacyanoferrate electrodeposited on a golden electrode was used as a mediaˉtor.The effects of assay conditions on the performances of the choline biosensor were studied.The optimum conditions were achieved with pH6.8and applied potential of-0.1V.The biosensor had a fast response with a linear calibration range of2.0×10 -5 -1.0×10 -3 mol/L and a detection limit of5.0×10 -6 mol/L.Both sensitivity and selectivity of the biosensor were excellent.The efficacy of the biosensor was75%of its initial value after one_month usage.
出处
《分析测试学报》
CAS
CSCD
北大核心
2005年第3期25-27,33,共4页
Journal of Instrumental Analysis
基金
湖南省自然科学基金资助项目(04JJ30047)
关键词
壳聚糖
铁氰酸镍
电沉积
辣根过氧化物酶
胆碱氧化酶
Chitosan
Nickel hexacyanoferrate
Electrodeposition
Horseradish peroxidase
Choline oxidase
