摘要
对水貂黑素皮质素受体-4(MC4R)基因进行了克隆和序列分析,以期为进一步开展水貂MC4R基因与其肥胖性状的相关分析及基因定位等研究提供理论基础。首先采用特定引物对水貂MC4R基因进行PCR扩增、克隆和测序,然后用DNAMAN软件拼接MC4R基因全序列,并进行序列的同源性分析。试验获得水貂MC4R基因序列816 bp。水貂MC4R基因序列与同一种属的水獭的同源性最高,达96%,而与犬、狐、大熊猫、河马、野猪、山羊、人及小鼠这些哺乳动物的同源性在89%～95%之间,另外,与贝加尔湖海豹和加州海狮的同源性也较高,均为94%。本研究成功克隆了水貂的MC4R基因,结果表明,其在物种间具有较高的保守性。
Melanocortin receptor-4（MC4R）gene in mink was cloned and sequenced,in order to provide theoretical foundation for further research of the corelevantion of MC4R gene in mink and fat traits,and location.MC4R gene of mink was amplified using particular primers,cloned by polymerase chain reaction（PCR） method and sequenced,and spliced by using DNAMAN.The length of MC4R gene in mink was 816 bp.The sequences had been sent to GenBank and registered.Comparison of the fragment sequence with that of other mammal animals MC4R gene was carried out through Blast in GenBank,the results showed that the homologies of the nucleotide sequence with river otter,dog,fox,panda,river horse,boar,goat,human and rat were 89% to 95%,MC4R gene of mink was successfully cloned in this study,indicating it was quite conservative among species.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期101-104,共4页
China Animal Husbandry & Veterinary Medicine
关键词
MC4R基因
克隆测序
序列分析
水貂
melanocortin receptor-4 gene
cloning and sequencing
sequence analysis
mink
