摘要
目的提高普通的单基因聚合酶链反应（ＰＣＲ）检测产毒性大肠杆菌（ＥＴＥＣ）的时效。方法以ＥＴＥＣ４４８１３（ＳＴｐ＋）、ＥＴＥＣ１９４４９（ＳＴｈ＋）和ＥＴＥＣ４４８１５（ＬＴ＋）三个标准株为模板，建立了检测产毒性大肠杆菌多重ＰＣＲ扩增系统。结果杂交工程株Ｈ１０９０７（ＳＴｐ＋），ＰＳＬＭ００４（ＳＴｈ＋）和ＰＭＭ０３０（ＬＴ＋），杂交的结果都是阳性，５４株菌株经ＰＣＲ检测，其中ＬＴ＋８株、ＳＴｐ＋２株、ＳＴｈ＋７株、ＬＴ＋＋ＳＴｐ＋１０株、ＬＴ＋＋ＳＴｈ＋１０株、ＳＴｐ＋＋ＳＴｈ＋４株和ＬＴ＋＋ＳＴｐ＋＋ＳＴｈ＋１３株。结论用一次ＰＣＲ扩增可检测和鉴别ＥＴＥＣ，比单基因ＰＣＲ更加快速、经济。
Objective In order to raise the efficiency of general single gene
amplification in detecting ETEC.Methods Three gene PCR method had been set up with three
standard bacteria of ETEC44815(STp +), ETEC19449(STh +) and ETEC44813(LT +).Results
H10907(STp +),PSLM004(STh +) and PMM030(LT +) had been hybrided with the probes
labeled by the polymerase chain reaction. The hybridization results of all strains were positive,
which demonstrated the speciality of the multiplex PCR method. Fifty four ETEC had been
detected by this three gene amplificatin system and divided into LT(8),
STp(2),STh(7),LT+STP(10),LT+STh(10),STp+STh(4) and LT+STp+STh(13).Conclusion The results
suggests that mutiplex PCR is more rapid and economical than single gene one in the
detection and identification of ETEC.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
1999年第2期88-90,共3页
Chinese Journal of Infectious Diseases
基金
全军"九五"指令性课题资助
关键词
聚合酶链反应
产毒性大肠杆菌
毒力基因
Polymerase chain reaction Hybridization
Enterotoxigenic Escherichia coli
