摘要
研究肿瘤坏死因子（ＴＮＦ）影响单核细胞与体外培养的牛脑微血管内皮细胞（ＢＣＭＥＣ）粘附，迁移的动力学过程及药物的保护作用．利用培养在胶原层上的单层内皮细胞，通过对粘附于内皮细胞单层上的单核细胞及胶原层中的单核细胞计数，测定单核细胞粘附及迁移．ＴＮＦ（１００ｋＵ·Ｌ－１）可促进单核细胞与ＢＣＭＥＣ的粘附及迁移，其促粘附与迁移作用具明显的时效关系．ＴＮＦ促粘附达最大效应时间为２ｈ，较对照提高１２０．７％；ＴＮＦ促迁移作用１ｈ达到坪值，提高９１．９％．药物戊烯氧呋豆素（ｉｍ－ｐｅｒａｔｏｒｉｎ，ＩＭＰ），己酮可可碱（ｐｅｎｔｏｘｉｆｙｌｉｎｅ，ＰＴＸ）抑制由ＴＮＦ引起的单核细胞与ＢＣＭＥＣ的粘附及迁移，在浓度为１，１０及１００μｍｏｌ·Ｌ－１时，其粘附抑制率分别为７３．１％，８７．７％，９３．３％和７３．６％，８７．２％，８９．０％；迁移抑制率分别为１７．４％，４３．４％，６８．１％和３７．７％，４９．３％，６２．３％．ＩＭＰ和ＰＴＸ能抑制单核细胞与ＴＮＦ诱导的ＢＣＭＥＣ的粘附及迁移．
Antagonistic effects of pentoxifylline(PTX) and imperatorin(IMP)on adhesion of monocytes to and migration through tumor necrosis factor (TNF) stimulated bovine cerebromicrovas cular endothelial cell(BCMEC) layer in vitro were studied. The adhesion and migration of bovine monocytes were measured using a three dimensional model system comprising BCMEC cultured on collagen gels in vitro. The results showed that prestimulation of BCMEC with TNF(100 kU·L 1 ) enhanced monocytes adhesion to and migration through TNF stimulated BCMEC layers. Both adhesion and migration of monocytes were increased with time. After 2 h or 1 h incubation of BCMEC with TNF, adhesion or migration reached maximum (120.7% and 91.9% respectively). At concentrations from 1 to 100 μmol·L 1 , both IMP and PTX were found to inhibit the monocyte adhesion and migration. Their maximal adhesion inhibitory effects were 93.3% and 89.0% at 100 μmol·L 1 , respectively, and their maximal migration inhibitory effects were 68.1% and 62.3% at 100 μmol·L 1 , respectively.
出处
《中国药理学与毒理学杂志》
CAS
CSCD
北大核心
1998年第4期291-294,共4页
Chinese Journal of Pharmacology and Toxicology
基金
国家自然基金
关键词
肿瘤坏死因子
PTX
动脉粥样硬化
单核细胞粘附
tumor necrosis factor
migration
pentoxifylline
imperatorin
atherosclerosis
monocytes
