摘要
目的观察栉孔扇贝(Chlamys farreri)裙边糖胺聚糖(SS-GAG)对体外培养的血管平滑肌细胞(VSMC)内原癌基因c-myc mRNA、肿瘤坏死因子(TNF-α)mRNA水平的影响,以探讨SS-GAG抗动脉粥样硬化(AS)的作用机理。方法建立碱性成纤维细胞生长因子(bFGF)诱导的平滑肌细胞增殖模型,采用非同位素原位杂交方法测定SS-GAG对增殖的VSMC内c-myc mRNA、TNFαmRNA水平的影响。结果低、高剂量SS-GAG组c-myc、TNF-αmRNA水平明显低于模型组(P<0.01)。结论SS-GAG对bFGF诱导增殖的VSMC内c-myc、TNF-α基因的表达均有抑制作用,且随着SS-GAG浓度的升高抑制作用增强。
Objective To observe the effects of Chlamys farreri skirt glycosaminoglycan（SS- GAG） on the expressions of c-myc and TNF-α genes so as to explore the anti-atherosclerosis （AS） mechanism of SS-GAG. Methods The cell proliferation model of vascular smooth muscle cells （VSMC） was established by basic fibroblast growth factor （bFGF） induetion. In Situ hybridization marked by non-isotope was applied to determine the effects of SS-GAG on the mRNA levels of c-myc and TNF in proliferative VSMC. Results The mRNA levels of c- myc and TNF in low dose group and high group of SS-GAG were obviously lower than that of model group （P〈0.01）. Conclusion SS-GAG has inhibitory effect on both c-myc and TNF gene expression in proliferative VSMC induced by bFGF and the effect is concentration dependent.
出处
《中国海洋药物》
CAS
CSCD
2008年第5期41-45,共5页
Chinese Journal of Marine Drugs
关键词
栉孔扇贝
糖胺聚糖
肿瘤坏死因子
动脉粥样硬化
原位杂交
Chlarnys farreri
glycosaminoglycan（SS-GAG）
TNF-α
atherosclerosis （AS）
in situ hybridization
