摘要
建立了用离子色谱法测定硫酸软骨素的方法。在６ｍｏｌ／Ｌ盐酸介质中将硫酸软骨素水解生成等摩尔的硫酸根离子，然后用ＡｎｉｏｎＨＣ离子色谱柱进行分离，以硼酸钠／葡萄糖酸钠淋洗液为流动相，流速１．５ｍｌ／ｍｉｎ，电导检测器检测。硫酸根离子在０．００１～０．０８０ｍｇ／ｍｌ浓度范围内线性关系良好，相关系数为０．９９９４，最低检测浓度为０．０００６ｍｇ／ｍｌ。样品中硫酸根离子的加样回收率为９８．５１％～９９．５２％，硫酸软骨素原料药和注射液及鲨鱼软骨粉胶囊中硫酸软骨素测定的相对标准偏差分别为０．２５％，１．０６％和１．９６％。
An ion chromatographic method for the determination of chondroitin sulfate was described Chondroitin sulfate was hydrolyzed in 6 mol/L hydrochloride condition to release equal mole of sulfate ion Using sodium borate/gluconate eluent at a flow rate of 1 5 ml/min as the mobile phase, the sulfate was separated on an Anion HC column and monitored by conductivity detector The linear range of sulfate was 0 001～0 080 mg/ml with a correlation coefficient of 0 9994 and the minimum detectable limit was 0 0006 mg/ml The recoveries of sulfate in samples by standard addition method were between 98 51% and 99 52% The relative standard deviations of samples were 0 25% for chondroitin sulfate, 1 06% for injectio chondroitin sulfate and 1 96% for capsuls of shark cartilage The developed method has been applied to the quatitation of total amount of chondroitin sulfate in various formulations of preparations
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1998年第5期358-361,共4页
Journal of China Pharmaceutical University
关键词
离子色谱法
硫酸软骨素
鲨鱼软骨粉
硫酸根离子
Ion chromatography
Chondroitin sulfate
Shark cartilage
Sulfate ion
Determination of content
