摘要
目的探讨脂肪酸成分分析方法对广东布鲁氏菌菌株进行分型的可能性,获得广东布鲁氏菌脂肪酸成分的本底资料。方法选择历年从广东不同地区分离到的29株布鲁氏菌进行了菌体脂肪酸成分分析,利用MIDI公司Sherlock系统的软件进行聚类分析。结果广东布鲁氏菌的主要脂肪酸成分为19:0cycloω8c酸、16:0酸、18:0酸,其中牛种菌的19:0cycloω8c酸含量最高,羊种次之,猪种最低,其中羊种和猪种布鲁氏菌19:0cycloω8c酸、18:0酸含量差异有统计学意义,1965年和近3年的羊种布鲁氏菌株19:0cycloω8c酸、18:0酸含量差异有统计学意义。结论菌株的脂肪酸成分稳定,但各种间脂肪酸含量存在差异,一定的欧氏距离时,可以在种的水平上区分布鲁氏菌的3个种。
To explore the possibility to type the Brucella strains isolated in Guangdong province with analytical method to detect the fatty acid components and to collect the basic data of fatty acid components of Brucella strains, 29 strains of Brucelia were selected for analysis on the bacterial fatty acid components and the cluster analysis on the collected data was performed with Sherlock analysis soft-ware （MIDI）. It was demonstrated that the main fatty acid components of Brucella strains isolated in Guangdong province were 19 ： 0 cycloω8c acid, 16 ： 0 acid and 18 ： 0 acid. The content of 19 ： 0cycloω8c acid was highest in B. abortus, followed by B. melitensis and lowest in B. suis. In addition, the content differences of 19 ： 0cycloω8c and 18 ： 0 acid between B. melitensis and Brucella suis were statistically significant; and that of 19 ： 0cycloω8c and 18 ： 0 acid between strains isolated in 1965 and those isolated in recent 3 years was statistically significant. It was also shown that the fatty acid components of Brucella strains were stable, but the contents of fatty acid components were different in different species. It is evident that at certain euclidean distance, 3 species of Brucella can be differentiated in species level.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2010年第2期131-133,139,共4页
Chinese Journal of Zoonoses
基金
广东省农业攻关计划(2006A20301006)资助
关键词
布鲁氏菌
脂肪酸
分析
Brucella
fatty acids
analytical method
