摘要
目的观察反义血管内皮生长因子C(antiVEGF-C)基因转染对人胃癌细胞系SGC-7901成瘤性和脉管生成的影响,探讨VEGF-C在脉管新生和胃癌转移中的作用。方法30只BALB/c裸鼠随机分为转染antiVEGF-C组、转染空白质粒组及未转染质粒组(n=10),采用皮下注射分别转染antiVEGF-C基因、转染空白质粒及未转染质粒的SGC-7901细胞悬液0.2ml(1×107/ml),每2d注射1次,连续3次,观察裸鼠皮下肿瘤的生成速度,并对肿瘤组织中的微淋巴管及微血管密度进行检测。结果转染antiVEGF-C组裸鼠肿瘤生长速度明显减慢且瘤体较小。注射后第1、2、3周,转染antiVEGF-C组肿瘤组织中微淋巴管密度分别为4.0±2.2、6.0±3.1、9.0±2.7每高倍视野(/HF),转染空白质粒组分别为6.0±8.7、9.0±3.5、18.0±7.2/HF,未转染质粒组分别为7.0±4.9、9.0±6.4、19.0±6.5/HF,转染antiVEGF-C组肿瘤组织中微血管密度分别为3.0±2.4、5.0±2.1、8.0±1.7/HF,转染空白质粒组分别为4.0±1.8、6.0±2.7、10.0±1.3/HF,未转染质粒组分别为4.0±1.5、6.0±1.3、9.0±1.2/HF。转染antiVEGF-C组肿瘤组织中新生微淋巴管较未转染质粒组及转染空白质粒组明显减少(P<0.05),但新生微血管的数量与其他两组比较差异无统计学意义(P>0.05)。结论antiVEGF-C转染对人胃癌细胞SGC-7901的成瘤性及淋巴管生成有明显抑制作用,但对肿瘤新生血管的形成影响不大。VEGF-C可能参与了胃癌新生淋巴管的形成过程。
Objective To investigate the biological effects of human gastric carcinoma cell line SGC-7901 transinfected by antisence vascular endothelial growth factor-C （anti VEGF-C） gene on the tumorigenicity and vascular generation,and explore the role of VEGF-C gene in metastasis and vascular generation of gastric cancer.MethodsThirty nude mice were randomly divided into three groups （10 each）：transgenic anti VEGF-C group,transgenic pure liposome group and non-transgenic group.0.2ml （1×107/ml） of SGC-7901 suspension,either transinfected by anti VEGF-C gene,or by pure liposome,or transinfected nil,was respectively subcutaneously injected into the three groups of mice,once every 2 days for 3 consecutive days.After injection,the size and growth velocity of the neoplasm were measured.The microlymphatic vessel density （MLVD） and microvessel density （MVD） of tumor were also determined.ResultsThe neoplasm grew more slowly and was smaller in the mice of transgenic anti VEGF-C group than in non-transgenic ones.At the end of the 1st,2nd and 3rd week,the MLVD in the mice of transgenic anti VEGF-C group was 4.0±2.2,6.0±3.1 and 9.0±2.7/high field （/HF）,respectively.In the transgenic pure liposome group,the MLVD was 6.0±8.7,9.0±3.5 and 18.0±7.2/HF,respectively.In the non-transgenic group,the MLVD was 7.0±4.9,9.0±6.4 and 19.0±6.5/HF,respectively.The MVD in the transgenic anti VEGF-C group was 3.0±2.4,5.0±2.1 and 8.0±1.7/HF,respectively.In the transgenic pure liposome group was 4.0±1.8,6.0±2.7 and 10.0±1.3/HF,respectively,and in non-transgenic group was 4.0±1.5,6.0±1.3 and 9.0±1.2/HF,respectively.There existed significant difference in lymphangiogenesis of the tumor （P0.05） between the transgenic anti VEGF-C group and the other two groups,but only little difference existed in angiogenesis of the tumor （P0.05）.ConclusionsThe anti VEGF-C gene may suppress the tumorigenicity and lymphangiogenesis of human SGC-7901 gastric carcinoma cells,but have little effect on angiogenesis of the tumor.VEGF-C might participate in the lymphangiogenesis of gastric tumor.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2010年第2期192-194,共3页
Medical Journal of Chinese People's Liberation Army
关键词
寡核糖核苷酸类
反义
淋巴管生成
肿瘤形成过程
胃肿瘤
oligoribonucleotides
antisense
lymphangiogenesis
neoplastic processes
stomach neoplasms
