摘要
目的:通过观察胃癌裸鼠移植瘤的生长过程,研究反义血管内皮生长因子-C(Anti-sense vascular endothelial growth factor-C,Anti-sense VEGF-C)基因转染胃癌细胞后对其在裸鼠的成瘤性和肿瘤脉管生成中的作用。方法:将30只裸鼠随机分成3组,将转染了anti-sense VEGF-C基因、转染空白质粒及未转染质粒的人胃癌细胞系SGC-79013种细胞的细胞悬液,分别接种于3组裸鼠皮下,使之在裸鼠皮下产生肿瘤结节,观察肿瘤生成的速度并对肿瘤组织中的微淋巴管及微血管进行检测。结果:转染anti-sense VEGF-C基因裸鼠肿瘤生长速度明显减慢且瘤体较小(P<0.05)。第1、2、3周,转染anti-sense VEGF-C组肿瘤组织中微淋巴管密度较其他2组明显减少(P<0.05):转染anti-sense VEGF-C组分别为4.0±2.2/HF、6.0±3.1/HF和9.0±2.7/HF;转染空白质粒组分别为6.0±8.7/HF、9.0±3.5/HF和18.0±7.2/HF;未转染质粒组分别为7.0±4.9/HF,9.0±6.4/HF和19.0±6.5/HF。但新生血管生成的数量3组比较差异无统计学意义(P>0.05)。结论:anti-sense VEGF-C转染对人胃癌细胞SGC-7901裸鼠成瘤性及肿瘤淋巴管生成有明显的抑制作用,但对肿瘤新生血管的形成影响不大。VEGF-C可能参与了胃癌新生淋巴管的形成过程。
Objective：To investigate the effects of tumorigenicity and vascular generation of nude mice on antisence vascular endothelial growth factor-C（antisense VEGF-C） gene transinfection of human gastric carcinoma cell by observing the vegetation process of implanted gastric tumor.Methods：30 nude mice were divided into three groups randomly：transgenic anti-sense VEGF-C group,transgenic pure liposome group and non-transgenic group.The cell suspension of gastric carcinoma cells SGC-7901 non-transinfected or transinfected by anti-sense VEGF-C gene or pure liposome were subcutaneously injected into those mice.After injection,the size and growth velocity of those neoplasma were detected.The Microlymphatic vessel density（MLVD） and microvessel density（MVD） of tumor were detected too.Results：The neoplasma grew more slowly and was smaller in the mice in those transinfected by anti-sense VEGF-C gene than in non-transgenic ones（P0.05）.At the end of the 1st,2nd and 3rd week,there was significant difference between the anti-sense VEGF-C group and the other groups on lymphangiogenesis of the tumor（P0.05）.the MLVD in the anti-sense VEGF-C group was 4±2.2/HF,6±3.1/HF and 9±2.7/HF respectively.In the transgenic pure liposome group,it was 6±8.7/HF,9±3.5/HF and 18±7.2/HF respectively.In the non-transgenic group,it was 7.0±4.9/HF,9.0±6.4/HF and 19.0±6.5/HF respectively.But it had little difference on angiogenesis of the tumor in the 3 groups（P0.05）.Conclusions：The antisense VEGF-C gene suppress the tumorigenicity and lymphangiogenesis in the human SGC-7901 gastric carcinoma cell apparently.but it had little effects on angiogenesis of the tumor.VEGF-C might participate the lymphangiogenesis of the gastric tumor.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第3期358-360,共3页
Journal of Chongqing Medical University
基金
重庆医科大学科研创新基金(编号:CX200503)
关键词
裸鼠
移植瘤
反义血管内皮生长因子-C
成瘤性
脉管生成
Nude mice
Transplantable tumor
Antisense vascular endothelial growth factor-C
Tumorigenicity
Vascular generation
