摘要
从13种离子交换树脂和吸附树脂中,筛选出固定化效果较好的大孔弱碱性苯乙烯系阴离子交换树脂D380为载体,以戊二醛为交联剂,通过先吸附后交联的方法对果糖基转移酶的固定化进行分析,并对固定化条件进行了优化。结果表明,最佳固定化条件为:加酶量为200U/g树脂,吸附pH为6.0,吸附时间为6h,吸附温度为30℃,交联剂戊二醛浓度为0.01%,交联时间为6h,交联温度为4℃,固定化酶活回收率最高可达87.6%以上。
Fructosyltransferase was immobilized on 13 kinds of ion-exchange and adsorption resins, among them D380 showed the excellent result for immobilization.Then the enzyme was immobilized through cross-linkage of glutaraldehyde.The optimum conditions for the immobilization were as follows：200U of enzyme per g wet resin, pH 6.0,30℃, and 6h, respectively. The crosslinking temperature and time were 4% and 6h respectively, and the concentration of the crosslinking agent （giutaraldehyde）was 0.01%.The activity yield of immobilized enzyme was above 87.6%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第2期174-177,共4页
Science and Technology of Food Industry
基金
重点实验室目标导向项目(SKLF-MB-200804)
关键词
果糖转移酶
固定化
树脂
fructosyltransferase
immobilization
resin
