摘要
以骨胶原蛋白为原料,采用微波酸处理辅助酶解制备胶原蛋白肽,以水解度及抗氧化能力为指标确定最佳水解条件。通过比较实验确定最佳微波酸处理条件为:微波功率510W作用270s;通过对酸性蛋白酶、木瓜蛋白酶、中性蛋白酶及碱性蛋白酶水解结果比较,确定中性蛋白酶水解产物的水解度及ABTS、DPPH自由基清除率最高;通过单因素实验及正交实验优化中性酶最佳酶解条件为:酶与底物比10%,底物浓度4%,反应温度55℃,pH7.0。结果表明,与单独酶解相比,微波酸处理能够使骨胶原蛋白酶解时间缩短1/2,水解度上升3.2%,产物的ABTS、DPPH自由基清除率分别提高8.7%和3.1%。
Collagen hydrolysate produced with microwave-acid treatment pretreatment combined with hydrolysis by enzyme.The optimum technology conditions were studied with the degree of hydrolysis and antioxidant activity as index.Through the comparison test,the optimum conditions for microwave treatment were determined as follows： microwave output power 510W, microwave radiation time 270s.Neutral protease was chosen as the best enzyme by the comparison of acid protease, papain, neutral protease and alkaline protease. The optimum hydrolysis technology for enzymolysis with neutral protease was confirmed through the orthogonal test. [E]/[ S ] = 10%, [ S] =4% ,tempreture 55℃, pH7.0.Compared with conventional enzymolysis,the reacting time was shortened by 1/2,degree of hydrolysis increased by 3.2%, ABTS and DPPH radical scavenging activity of hydrolysate were increased by 8.7% and 3.1% respectively.
出处
《食品工业科技》
CAS
CSCD
北大核心
2010年第2期97-100,共4页
Science and Technology of Food Industry
基金
863计划项目(2007AA10Z325)
科技部十一五支撑计划项目(2006BAD27)
关键词
骨胶原蛋白
微波酸水解
酶解
水解度
抗氧化
collagen
microwave-acid hydrolysis
enzymolysis
degree of hydrolysis
antioxidant activity
