摘要
为了分离组织特异性、诱导性的启动子,本研究根据新克隆的浮萍rbcS基因序列设计引物,采用改进的5'walking技术从浮萍基因组中克隆了一个新的rbcS基因启动子,命名为SSU5C基因启动子。序列分析表明:SSU5C基因启动子长度为1543bp,含有保守性元件TATA和CAATbox,并且含有水杨酸、脱落酸、赤霉素、茉莉酸甲酯等植物激素和葡萄糖等的保守顺式作用元件。Insilico分析初步推测SSU5C基因启动子受多种信号途径的协调调控。
In order to isolate the tissue-specific and inducible promoter, we designed primers based on the DNA sequence of a newly cloned rbcS gene in duckweed, and cloned a novel rbcS promoter by using improved 5＇ walking technique, named as SSU5C promoter. Sequence analysis showed that the length of SSU5C promoter is 1 543 bp, it consisted of TATA and CAAT box, as well as cis-acting element of plant hormone, such as SA, ABA, GA, SA, and glucose etc. We preliminary predicted by in silico ananlysis that SSU5C promoter might be coordinately regulated by multiple signaling pathways.
出处
《分子植物育种》
CAS
CSCD
2010年第1期41-44,共4页
Molecular Plant Breeding
