摘要
从细茎大豆的嫩叶中提取总ＤＮＡ，用ＰＣＲ方法扩增得到包含完整编码区的 ｒｂｃ Ｓ基因并将其克隆到ＰＢＬＵＥＳＣＲＩＰＴ载体中．完成全基因 １０８９个核苷酸测序后，运用ＰＣ－ＧＥＮＥ进行顺序编辑和同源比较，并应用ＭＥＧＡ１．０２软件中的Ｎｅｉｇｈｂｏｒ－Ｊｏｉｎｉｎｇ方法画出Ｒｕｂｉｓｃｏ小亚基分子的系统进化树．
Total DNA was extracted from tender leaves of semi-cultural soybean (G. gracilis). The rbcS containing the whole coding region for Rubisco small subunit was amplified by PCR and then cloned and sequenced. This gene was subjected to homology analysis from PCGENE package, and the phylogenetic tree of Rubisco small subunit from different plants was constructed by neighbor-joining method of MEGA 1. 02 package.
出处
《复旦学报（自然科学版）》
CAS
CSCD
北大核心
1998年第2期151-156,共6页
Journal of Fudan University：Natural Science
基金
国家自然科学基金
关键词
大豆
序列分析
分子进化
rbcs基因
细茎大豆
soybean
rbcS
sequence analysis
neighbor-joining method
molecular evolution
