摘要
目的探讨EphA2/EphrinA1在肾癌中的表达及其与肾癌临床病理特征的关系。方法采用半定量逆转录聚合酶链反应(RT-PCR)和流式细胞术检测22例肾癌组织和12例非肾癌正常肾组织中EphA2、EphrinA1的基因及蛋白表达情况,并对其进行比较和相关分析;利用免疫组织化学法检测68例肾癌组织和24例正常肾组织中EphA2、EphrinA1的表达,分析与肾癌临床病理特征之间的关系。结果肾癌组织中EphA2和EphrinA1的mRAN和蛋白均明显高于正常肾组织(1.60±0.80 vs 0.58±0.19,1.37±0.63 vs 0.91±0.40;424.38±43.14 vs 332.44±42.83,419.44±52.13 vs 325.36±42.33,均P<0.01)。但两种蛋白与其mRAN表达均不存在相关关系(r=0.050,P>0.05;r=-0.171,P>0.05)。在肾癌组织分级不同的各组间,EphA2、EphrinA1蛋白的表达差异有统计学意义(χ2=9.575,P<0.01;χ2=7.947,P<0.05),且随着等级的增加,EphA2、EphrinA1蛋白的表达量也显著增加(r=0.350,P<0.01;r=0.344,P<0.01)。结论EphA2和EphrinA1蛋白高表达于肾癌高分期组、高分级组和淋巴结转移组,其高表达的机制除与基因表达上调有关外,EphA2蛋白降解受阻可能是导致其升高的重要因素。
Objective To detect the expression of EphA2/EphrinA1 in renal cell carcinoma （RCC） and its relationship with clinicopathological characteristics. Methods Reversed transcript-polymerase chain reaction（RT-PCR） and flow cytometry were used to detect mRNA of the EphA2 and EphrinAl,and the protein expression in 22 cases of RCC and 12 cases of normal renal tissue. EphA2/EphrinA1 expression were detected using immunohistochemistry in 68 cases of RCC and 24 eases of normal renal tissue. The relationship of EphA2/EphrinA1 expression with clinicopathological features were explored. Results The expression of the EphA2 mRNA, EphrinA1 mRNA and protein in the RCC were higher than those in the normal renal tissue （1.60±0.80 vs 0. 58±0.19,1. 37±0. 63 vs 0.91±0.40 ;424.38±43.14 vs 332.44±42.83,419.44±52.13 vs 325.36±42.33;all P 〈0.01 ）, but correlation did not exist in the RCC,EphA2/EphrinA1 protein expression and its mRNA expression （ r =0.05, P 〉0.05; r =0. 171, P 〉0.05）. In the RCC,different grades had different protein expressions of EphA2/EphrinAl（X^2 =9. 575, P 〈0.01; X^2=7. 947, P 〈0.05）. With the increase in grade levels, the protein expression of EphA2/EphrinA1 increased significantly（ r=0.350, P 〈0.011; r =0.344, P 〈0.01）. Conclusion EphA2/EphrinA1 is highly expressed in tumors with high stage, high grade and with lymph nodes metastasis. The binding of the EphA2 and EphrinA1 may inhibit the proliferatin of the tumor. In the RCC, EphA2/EphrinA1 protein expression is not only associated with the high gene expression, but also with the post transcriptional regulation and the biological abnormalities of EphA2 / EphrinA1.
出处
《临床荟萃》
CAS
2010年第4期295-298,F0003,共5页
Clinical Focus
关键词
肾肿瘤
受体蛋白酪氨酸激酶
聚合酶链反应
renal neoplasms
receptor protein tyrosine kinase
polymerase chain reaction
