摘要
目的探讨血清人肝特异性 F 抗原作为肝脏功能指标在肝病诊断中的价值。方法生物素-抗生物素蛋白酶联免疫吸附法(BA-ELISA)测定各类肝病患者血清中人肝特异性 F 抗原。结果 BA-ELISA 方法灵敏、稳定。肝病血清 F-Ag 阳性率为85.2%(46/54);对慢性肝炎、肝硬化和肝癌等的诊断价值高于天冬氨酸转氨酶(AST);肝癌血清 F-Ag 的检出率为91.3%(21/23),与甲胎蛋白含量具有相关性。结论 F-Ag 可以作为判断肝损伤和肝脏功能状况的指标,对肝病尤其是肝癌的诊断具有明显的临床实用价值。
Objectives To investigate the significance of F-Ag as the specific marker of hepatocellular damage and the value in the diagnosis of liver disease.Methods A biotin-avidin ELISA (BA-ELISA)was developed for the measurement of human liver-specific F antigen(F-Ag)in sera of patients with various liver diseases.Results BA-ELISA were highly sensitive.F-Ag was detectable in 46 of 54(85.2%)patients with various liver diseases and 21 of 23(91.3%)hepatocellular carcinoma, showed a close correlation with AST in acute hepatitis and AFP in hepatocellular carcinoma. Conclusion The serum F-Ag concentration was a more sensitive and specific marker of liver damage than conventional liver function tests.The use of F-Ag may facilitate detection of hepatocellular carcinoma in some patients.
出处
《中华肝脏病杂志》
CAS
CSCD
1998年第3期170-171,共2页
Chinese Journal of Hepatology
基金
四川省教委资助
