摘要
目的观察中药复方解聚复肾宁(JJFSN)对糖尿病(DM)大鼠肾脏的保护作用。方法建立链脲佐菌素(STZ)诱导的DM大鼠模型,将成模大鼠随机分成4组:模型组、JJFSN组、厄贝沙坦组、JJFSN+厄贝沙坦组,并设正常对照组。各组大鼠采用相应的干预措施处理12w。常规方法检测各组大鼠尿白蛋白排泄率(UAER)等指标,免疫组化法检测肾血管内皮生长因子(VEGF)和骨形成蛋白7(BMP-7)表达,Western印迹检测肾组织血小板衍化生长因子B(PDGF-B)的表达,透射电镜观察肾脏超微结构。结果模型组大鼠除肾BMP-7表达显著减少外,其余指标均显著增高,肾脏超微结构改变明显;JJFSN组、厄贝沙坦组和JJFSN+厄贝沙坦组除肾BMP-7显著高于模型组(P<0.05)外,其余指标均显著低于模型组(P<0.05),肾脏超微结构改变明显改善;JJFSN+厄贝沙坦组各项指标改善明显优于模型组、JJFSN组和厄贝沙坦组(P<0.05),但未达到正常对照组水平。结论JJFSN对DM大鼠肾脏有明显保护作用。
Objective To investigate the effect of Jiejufushenning（JJFSN） on renal protection in diabetic nephropathy rats. Methods The diabetic SD rats induced by STZ were divided into 4 groups： model, JJFSN, irbesartan and JJFSN combined with irbesartan and control group. Rats in each group were respectively treated for 12 weeks by the appropriate intervention methods. The relative urinary albumin excretion rate （UAER） , and other indicators were detected by routine analysis methods, and VEGF and BMP-7 in renal tissue were detected by immunohistochemical techniques, and PDGF-B in renal tissue was detected by Western blot, and ultramicrostructure of kidney was observed by transmission electron microscope. Results In addition to renal cortex BMP-7 expression of a significant reduction, the other indicators were significantly higher in diabetic rats. The ultramicrostructure of kidney was noticeably changed. While the above indexes in JJFSN, irbesartan and JJFSN combined with irbesartan groups were significantly better than in diabetic control group （ P 〈 0. 05 ）, and ultramicrostructure of kidney changed was improved, and the improvement of all indexes in JJFSN combined with irbesartan group was most significant （P 〈 0. 05）. Conclusions Protective effect of JJFSN on diabetic nephropathy in rats is obvious.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2010年第1期46-49,共4页
Chinese Journal of Gerontology
