摘要
[目的]建立检测细胞培养物中支原体快速有效的方法。[方法]从6种常见污染细胞的支原体16sRNA中选择2段高度保守的核酸序列作为引物,用PCR和DNA荧光染色法来检测实验室中25种动物细胞株。[结果]用DNA荧光染色法检测实验室中25种动物细胞株阳性率为24%,可疑阳性为16%;而用PCR法检测阳性率为36%。[结论]PCR法较DNA荧光染色法灵敏度更高、特异性更强,将2种方法结合应用效果更好。
[ Objective] To establish a rapid and efficient method for detecting the mycoplasma contamination of cell cultures . [ Method] Two highly conserved nucleotide sequences were chosen as coplasma＇ primers from 16sRNA in the six common kinds of mycoplasma contaminated cells. PCR and DNA fluorescence staining method were selected to detecte 25 kinds of animals cell lines in laboratory. [ Result] The positive rate is 24% by DNA fluorescence staining and the suspicious positive is 16%. The positive rate is 36% by PCR, [ Conclusion] The PCR method has a higher sensitivity and a greater specificity than DNA fluorescence staining. The effect of the combined use them is better.
出处
《安徽农业科学》
CAS
北大核心
2010年第3期1151-1153,共3页
Journal of Anhui Agricultural Sciences
基金
安徽省自然科学基金研究重点项目(KJ2008A085)
安徽省科技攻关项目(08010302179)
2008年国家自然科学基金面上项目(30872253)
